Richtlijnen voor validatie van kwalitatieve real-time PCR methoden

Sylvia Broeders, Ingrid Huber, Lutz Grohmann, Gilbert Berben, Isabel Taverniers, Marco Mazzara, Nancy Roosens, Dany Morisset

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.
    TaalEngels
    TijdschriftTrends in Food Science and Technology
    Volume37
    Exemplaarnummer2
    Pagina's (van-tot)115-126
    Aantal pagina's12
    ISSN0924-2244
    StatusGepubliceerd - 30-jun-2014

    Trefwoorden

    • B420-voeding

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