Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion onL-lysine monohydrochloride produced by fermentation with the genetically modified strain of Corynebacterium glutamicum DSM 32932. Neither the production strain nor its recombinant DNA were detected in the final product. The additive does not pose any safety concern associated with the genetic modification of the production strain.L-Lysine HCl produced by C. glutamicum DSM 32932 is considered safe for the target species, for the consumer and for the environment.L-Lysine HCl produced by C. glutamicum DSM 32932 is not toxic by inhalation; it is not irritant to skin and not a skin sensitiser. The additive is not corrosive to eyes but it should be considered as an eye irritant. In general,L-lysine HCl is considered as an efficacious source of the essential amino acidL-lysine for non-ruminant animal species. For the supplementalL-lysine to be as efficacious in ruminants as in non-ruminant species, it would require protection against degradation in the rumen.