TY - JOUR
T1 - Screening for antimicrobial residues in poultry eggs in Bangladesh using Charm II radio-receptor assay technique following validation
AU - Chakma, Darmin
AU - Billah Prince, Md. Maruf
AU - Islam, Md. Hedayetul
AU - Tanvir, E.M.
AU - Nabi, Md. Mahamodun
AU - Reybroeck, Wim
AU - Sasanya, James Jacob
AU - Islam, M. Jahurul
AU - Islam, Md. Nazrul
AU - Talukder, Md. Shahabe Uddin
AU - Shaha, Chironjit Kumar
AU - Mannan, M.A.
AU - Saifullah, A.S.M.
PY - 2023/6/28
Y1 - 2023/6/28
N2 - The aim of the study was to screen for the presence of antimicrobial residues in poultry eggs from Bangladesh using the Charm II radio-receptor assay in the absence of expensive confirmatory instrumentation. This was based on cut-off values as set in the validation guidelines according to Commission Decision 2002/657/EC and Commission Implementing Regulation (EU) 2021/808. Fortified eggs spiked with fixed concentrations of doxycycline, erythromycin A, sulphamethazine, and benzylpenicillin were used to determine the cut-off values and detection capabilities (CCβ). Other validation parameters included were applicability, ruggedness, and robustness. A total of 201 egg mix samples from native organic chicken, duck, and commercial farm-raised laying hens (both brown and white eggs) were tested and after analysis 13%, 10%, and 4.5% of the egg mix samples showed positive signals for sulphonamides, macrolides/lincosamides, and tetracyclines, respectively. Presence of multiple drug residues were also suspected in 11 out of 201 egg mix samples.
AB - The aim of the study was to screen for the presence of antimicrobial residues in poultry eggs from Bangladesh using the Charm II radio-receptor assay in the absence of expensive confirmatory instrumentation. This was based on cut-off values as set in the validation guidelines according to Commission Decision 2002/657/EC and Commission Implementing Regulation (EU) 2021/808. Fortified eggs spiked with fixed concentrations of doxycycline, erythromycin A, sulphamethazine, and benzylpenicillin were used to determine the cut-off values and detection capabilities (CCβ). Other validation parameters included were applicability, ruggedness, and robustness. A total of 201 egg mix samples from native organic chicken, duck, and commercial farm-raised laying hens (both brown and white eggs) were tested and after analysis 13%, 10%, and 4.5% of the egg mix samples showed positive signals for sulphonamides, macrolides/lincosamides, and tetracyclines, respectively. Presence of multiple drug residues were also suspected in 11 out of 201 egg mix samples.
UR - https://www.mendeley.com/catalogue/807ad3e8-ce79-3c87-bb59-21a424bd81fb/
U2 - 10.1080/19393210.2023.2225157
DO - 10.1080/19393210.2023.2225157
M3 - A1: Web of Science-article
SP - 1
EP - 13
JO - Food Additives & Contaminants: Part B
JF - Food Additives & Contaminants: Part B
ER -