Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA

A-C Huet, L Mortier, E Daeseleire, T Fodey, C Elliott, P Delahaut

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was <0.5 microg kg(-1) in egg and <1 microg kg(-1) in muscle. For dinitrocarbanilide, the CCbeta was estimated at <3 microg kg(-1) in egg and <10 microg kg(-1) in chicken muscle.
    TaalEngels
    TijdschriftFood Additives and Contaminants
    Volume22
    Exemplaarnummer2
    Pagina's (van-tot)128-134
    Aantal pagina's7
    ISSN0265-203X
    DOI's
    StatusGepubliceerd - 2005

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