Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed

L E J Peeters; T De Mulder; E Van Coillie; J Huygens; A Smet; E Daeseleire; J Dewulf; H Imberechts; P Butaye; F Haesebrouck; S Croubels; M Heyndrickx; G Rasschaert

doi:10.1111/jam.13561

Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed

L E J Peeters, T De Mulder, E Van Coillie, J Huygens, A Smet, E Daeseleire, J Dewulf, H Imberechts, P Butaye, F Haesebrouck, S Croubels, M Heyndrickx, G Rasschaert

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

Uittreksel

AIMS: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l-1 ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum.

METHODS AND RESULTS: A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l-1 DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n = 454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4 mg l-1 DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4 mg l-1 DOX compared to medium without DOX.

CONCLUSIONS: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E. coli, and promote the selection of transconjugants in a porcine caecum model.

SIGNIFICANCE AND IMPACT OF THE STUDY: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.

Oorspronkelijke taal	Engels
Tijdschrift	Journal of Applied Microbiology
Volume	123
Exemplaarnummer	5
Pagina's (van-tot)	1312-1320
Aantal pagina’s	9
ISSN	1364-5072
DOI's	https://doi.org/10.1111/jam.13561
Publicatiestatus	Gepubliceerd - nov-2017

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10.1111/jam.13561

1 Afgerond

CROSSCONTAM: Antimicrobiële resistentie - Impact van kruisbesmetting van diervoeders met antimicrobiële middelen op de resistentieontwikkeling
Heyndrickx, M., Daeseleire, E., Rasschaert, G. & Van Coillie, E.
1/01/13 → 28/02/17
Project: Onderzoek

1 Andere soorten (prijzen, externe en andere activiteiten) - (Co)promotor doctoraatsthesis

Carry-over of tetracyclines and sulphonamides-trimethoprim in pig feed, resistance selection in the pig microbiota caused by doxycycline (Laura Peeters)
Geertrui Rasschaert (Copromotor)
2013 → 19-feb-2018
Activiteit: Overige › Andere soorten (prijzen, externe en andere activiteiten) - (Co)promotor doctoraatsthesis

Dit citeren

Peeters, L. E. J., De Mulder, T., Van Coillie, E., Huygens, J., Smet, A., Daeseleire, E., Dewulf, J., Imberechts, H., Butaye, P., Haesebrouck, F., Croubels, S., Heyndrickx, M., & Rasschaert, G. (2017). Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed. Journal of Applied Microbiology, 123(5), 1312-1320. https://doi.org/10.1111/jam.13561

Peeters, L E J ; De Mulder, T ; Van Coillie, E ; Huygens, J ; Smet, A ; Daeseleire, E ; Dewulf, J ; Imberechts, H ; Butaye, P ; Haesebrouck, F ; Croubels, S ; Heyndrickx, M ; Rasschaert, G. / Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed. In: Journal of Applied Microbiology. 2017 ; Vol. 123, Nr. 5. blz. 1312-1320.

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title = "Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed",

abstract = "AIMS: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l-1 ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum.METHODS AND RESULTS: A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l-1 DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n = 454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4 mg l-1 DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4 mg l-1 DOX compared to medium without DOX.CONCLUSIONS: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E. coli, and promote the selection of transconjugants in a porcine caecum model.SIGNIFICANCE AND IMPACT OF THE STUDY: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.",

keywords = "Journal Article",

author = "Peeters, {L E J} and {De Mulder}, T and {Van Coillie}, E and J Huygens and A Smet and E Daeseleire and J Dewulf and H Imberechts and P Butaye and F Haesebrouck and S Croubels and M Heyndrickx and G Rasschaert",

note = "{\textcopyright} 2017 The Society for Applied Microbiology.",

year = "2017",

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doi = "10.1111/jam.13561",

language = "English",

volume = "123",

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Peeters, LEJ, De Mulder, T, Van Coillie, E, Huygens, J, Smet, A, Daeseleire, E, Dewulf, J, Imberechts, H, Butaye, P, Haesebrouck, F, Croubels, S, Heyndrickx, M & Rasschaert, G 2017, 'Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed', Journal of Applied Microbiology, vol. 123, nr. 5, blz. 1312-1320. https://doi.org/10.1111/jam.13561

Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed. / Peeters, L E J; De Mulder, T; Van Coillie, E; Huygens, J; Smet, A; Daeseleire, E; Dewulf, J; Imberechts, H; Butaye, P; Haesebrouck, F; Croubels, S; Heyndrickx, M ; Rasschaert, G.

In: Journal of Applied Microbiology, Vol. 123, Nr. 5, 11.2017, blz. 1312-1320.

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

TY - JOUR

T1 - Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed

AU - Peeters, L E J

AU - De Mulder, T

AU - Van Coillie, E

AU - Huygens, J

AU - Smet, A

AU - Daeseleire, E

AU - Dewulf, J

AU - Imberechts, H

AU - Butaye, P

AU - Haesebrouck, F

AU - Croubels, S

AU - Heyndrickx, M

AU - Rasschaert, G

PY - 2017/11

Y1 - 2017/11

N2 - AIMS: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l-1 ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum.METHODS AND RESULTS: A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l-1 DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n = 454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4 mg l-1 DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4 mg l-1 DOX compared to medium without DOX.CONCLUSIONS: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E. coli, and promote the selection of transconjugants in a porcine caecum model.SIGNIFICANCE AND IMPACT OF THE STUDY: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.

AB - AIMS: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l-1 ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum.METHODS AND RESULTS: A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l-1 DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n = 454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4 mg l-1 DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4 mg l-1 DOX compared to medium without DOX.CONCLUSIONS: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E. coli, and promote the selection of transconjugants in a porcine caecum model.SIGNIFICANCE AND IMPACT OF THE STUDY: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.

KW - Journal Article

U2 - 10.1111/jam.13561

DO - 10.1111/jam.13561

M3 - A1: Web of Science-article

C2 - 28799283

VL - 123

SP - 1312

EP - 1320

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -

Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed

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Projecten

CROSSCONTAM: Antimicrobiële resistentie - Impact van kruisbesmetting van diervoeders met antimicrobiële middelen op de resistentieontwikkeling

Activiteiten

Carry-over of tetracyclines and sulphonamides-trimethoprim in pig feed, resistance selection in the pig microbiota caused by doxycycline (Laura Peeters)

Dit citeren