Test performance study of isothermal amplification tests for the detection of grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’

Natasa Mehle, Polona Kogovsek, Fiona Constable, Kris De Jonghe, Marianne Loiseau, Flavio Veratti, Cristina Marzachi, Lucca Ferretti, Elisa Angelini, Luisa Filippin, Esmeraldina Sousa, Eugenia Maria de Andrade, Jennifer Hodgetts, Matt Dickinson, Marina Dermastia

    Onderzoeksoutput: Bijdrage aan tijdschriftA2: Artikel in een internationaal wetenschappelijk tijdschrift met peer review, dat niet inbegrepen is in A1peer review

    Uittreksel

    This test performance study (TPS) was carried out on DNA samples from grapevine, clema-tis, fungi and bacteria to compare and validate loop-mediated isothermal amplification(LAMP) tests for detection of Grapevine flavescence doree phytoplasma and ‘CandidatusPhytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevineflavescence doree phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed byKogovsek and colleagues), with proven applicability for rapid laboratory or on-site detectionwere included in this study. They were performed in 10 laboratories. In addition, the com-mercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence doree phy-toplasma was performed in three laboratories. The accuracy of the three tests was shown tobe over 98%. Moreover, the high accuracy of these tests, which used different devicesacross different laboratories, confirmed their reproducibility.
    Oorspronkelijke taalEngels
    TijdschriftEPPO Bulletin
    Volume47
    Exemplaarnummer1
    Pagina's (van-tot)18-23
    Aantal pagina’s6
    ISSN0250-8052
    DOI's
    PublicatiestatusGepubliceerd - 24-jan-2017

    Trefwoorden

    • B390-fytopathologie

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