The brown shrimp (Crangon crangon L.) ecdysteroid receptor complex: cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient Drosophila cell line

Yves Verhaegen, Koen Parmentier, Luc Swevers, Pierre Rougé, Thomas Soin, Wim De Coen, Kris Cooreman, Guy Smagghe

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

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    cDNAs encoding ecdysteroid receptor (EcR) and retinoid X receptor (RXR) were cloned and sequenced from brown shrimp Crangon crangon (Crustacea: Decapoda), a common faunal species and commercially important in the North-West European coastal waters. A 3D model of the ligand-binding domain (LBD) of EcR was created and docking of ponasterone A (PonA) was simulated in silico. Finally, we report the transfection of expression plasmids for these receptors in the mutant Drosophila L57-3-11 cell line. Through an ecdysteroid responsive reporter assay we clearly prove the functionality of shrimp ecdysteroid receptor in the transfected L57-3-11 cell line. Our results indicate that the Drosophila L57-3-11 cell line and in silico LBD modeling can be used to study the function of crustacean ecdysteroid receptors and be applied to assess endocrine disrupting effects on non-target crustacean species.
    TaalEngels
    TijdschriftGeneral and Comparative Endocrinology
    Volume168
    Exemplaarnummer3
    Pagina's (van-tot)415-23
    Aantal pagina's9
    ISSN0016-6480
    DOI's
    StatusGepubliceerd - 2010

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