The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth

Annemie Van Minnebruggen, Pia Neyt, Steven De Groeve, Griet Coussens, María Rosa Ponce, José Luis Micol, Mieke Van Lijsebettens

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review


    The role of translation in the regulation of higher plant growth and
    development is not well understood. Mutational analysis is a powerful tool
    to identify and study the function of genes related to a biological process,
    such as growth. Here we analyzed functionally the angusta3 (ang3) narrow
    leaf mutant. The ANG3 gene was cloned by fine mapping combined with
    candidate gene sequencing and it corresponded to the ribosomal protein gene
    RPL5B. Based on amino acid sequence homology, promoter DNA sequence
    homology and in silico gene expression analysis, RPL5B was found to be
    putatively functionally redundant with RPL5A. The morphological analysis of
    ang3 mutants showed that the leaf lamina area was significantly reduced from
    the third rosette leaf on, mainly because of decreased width. Cellular analysis
    of the abaxial epidermal cell layer of the third leaf indicated that the cell
    number in the mutant was similar to that of the wild type, but the cell size was
    significantly reduced. We postulate that the reduced cell expansion in the
    epidermis contributes to the narrow shape of ang3 leaves. Growth was also
    significantly impaired in hypocotyls and primary roots, hinting at a general
    role for RPL5B in organ growth, unrelated to dorsiventral axis formation.
    Comparison of the transcriptome of the shoot apices of the mutant and the
    wild type revealed a limited number of differentially expressed genes, such
    as MYB23 and MYB5, of which the lower expression in the ang3 mutant
    correlated with reduced trichome density. Our data suggest that translation is
    an important level of control of growth and development in plants.

    Oorspronkelijke taalEngels
    TijdschriftPhysiologia Plantarum
    Pagina's (van-tot)91-101
    Aantal pagina’s11
    PublicatiestatusGepubliceerd - 2010


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