The extensin signal peptide allows secretion of a heterologous protein from protoplasts

M De Loose, G Gheysen, C Tiré, J Gielen, R Villarroel, C Genetello, M Van Montagu, A Depicker, D Inzé

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    Extensins are hydroxyproline-rich glycoproteins which are amongst the most abundant proteins present in the cell wall of higher plants. Here, we describe the structural analysis of an extensin-encoding gene from Nicotiana plumbaginifolia. The encoded protein (46 kDa) has a highly repetitive structure and contains 37% proline, 18.1% tyrosine, 13.4% lysine, 8.1% serine and 7.1% histidine. The extensin-encoding sequence contains a typical signal peptide for translocation of the protein to the endoplasmic reticulum. By using chimeric genes consisting of different 5' parts of the extensin-encoding gene and the neomycin phosphotransferase II-encoding gene (nptII) as reporter gene, we show that the N-terminal part of extensin can mediate the secretion of NPTII from electroporated N. tabacum protoplasts.
    TaalEngels
    TijdschriftGene
    Volume99
    Exemplaarnummer1
    Pagina's (van-tot)95-100
    Aantal pagina's6
    ISSN0378-1119
    StatusGepubliceerd - 1991

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