Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

Hongli Ji, Godelieve Gheysen, Simon Denil, Keith Lindsey, Jennifer F Topping, Kamrun Nahar, Annelies Haegeman, Winnok H De Vos, Geert Trooskens, Wim Van Criekinge, Tim De Meyer, Tina Kyndt

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

    Uittreksel

    One of the reasons for the progressive yield decline observed in aerobic rice production is the rapid build-up of populations of the rice root knot nematode Meloidogyne graminicola. These nematodes induce specialized feeding cells inside root tissue, called giant cells. By injecting effectors in and sipping metabolites out of these cells, they reprogramme normal cell development and deprive the plant of its nutrients. In this research we have studied the transcriptome of giant cells in rice, after isolation of these cells by laser-capture microdissection. The expression profiles revealed a general induction of primary metabolism inside the giant cells. Although the roots were shielded from light induction, we detected a remarkable induction of genes involved in chloroplast biogenesis and tetrapyrrole synthesis. The presence of chloroplast-like structures inside these dark-grown cells was confirmed by confocal microscopy. On the other hand, genes involved in secondary metabolism and more specifically, the majority of defence-related genes were strongly suppressed in the giant cells. In addition, significant induction of transcripts involved in epigenetic processes was detected inside these cells 7 days after infection.
    Oorspronkelijke taalEngels
    TijdschriftJournal of Experimental Botany
    Volume64
    Exemplaarnummer12
    Pagina's (van-tot)3885-98
    Aantal pagina’s14
    ISSN0022-0957
    DOI's
    PublicatiestatusGepubliceerd - 2013

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