Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Asad Maroufi, Erik Van Bockstaele, Marc De Loose

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Uittreksel

Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.
TaalEngels
TijdschriftBMC Molecular Biology
Volume11
Pagina's (van-tot)15
ISSN1471-2199
DOI's
StatusGepubliceerd - 2010

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