Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Wim Reybroeck; Sigrid Ooghe

Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › C1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

Uittreksel

The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC.
All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL.
The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests.
Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

Oorspronkelijke taal	Engels
Titel	Residues of veterinary drugs in food : Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012
Editors	Robert Schilt
Aantal pagina’s	6
Volume	2
Publicatiedatum	2012
Pagina's	563-568
ISBN van geprinte versie	978-94-6173-318-4
Publicatiestatus	Gepubliceerd - 2012
Evenement	EuroResidue VII Conference on Residues of Veterinary Drugs in Food - Egmond aan Zee, Egmond aan Zee, Nederland Duur: 14-mei-2012 → 16-mei-2012

1 Afgerond
1 Actief

VALIDATIEANTIBIO: Validatie van screeningstesten voor antibioticaresiduen voor kitproducenten
Reybroeck, W. (Projectbegeleider), Heyndrickx, M. (Projectverantwoordelijke) & Ooghe, S. (Projectbegeleider)
1/01/10 → …
Project: Onderzoek
DETTECH: Ontwikkeling en validatie van nieuwe methoden voor detectie van residuen.
Reybroeck, W. (Projectverantwoordelijke) & Ooghe, S. (Onderzoeker)
1/01/95 → 31/12/17
Project: Onderzoek

Dit citeren

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title = "Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk",

abstract = "The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All {\ss}-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test). ",

author = "Wim Reybroeck and Sigrid Ooghe",

year = "2012",

language = "English",

isbn = "978-94-6173-318-4",

volume = "2",

pages = "563--568",

editor = "Robert Schilt",

booktitle = "Residues of veterinary drugs in food",

note = "EuroResidue VII Conference on Residues of Veterinary Drugs in Food ; Conference date: 14-05-2012 Through 16-05-2012",

}

Reybroeck, W & Ooghe, S 2012, Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk. in R Schilt (ed.), Residues of veterinary drugs in food: Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012. vol. 2, blz. 563-568, EuroResidue VII Conference on Residues of Veterinary Drugs in Food, Egmond aan Zee, Nederland, 14/05/12.

Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk. / Reybroeck, Wim ; Ooghe, Sigrid.
Residues of veterinary drugs in food: Proceedings of the EuroResidue VII Conference, Egmond aan Zee, The Netherlands, 14-16 May 2012. ed. / Robert Schilt. Vol. 2 2012. blz. 563-568.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › C1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

TY - CHAP

T1 - Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

AU - Reybroeck, Wim

AU - Ooghe, Sigrid

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N2 - The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

AB - The Charm Blue Yellow II (Charm Sciences Inc., Lawrence, MA) is a broad-spectrum microbiological inhibitor test for the detection of antimicrobials in milk. The test was validated at ILVO-T&V according to Commission Decision 2002/657/EC. All ß-lactam antibiotics were detected at their respective MRL, except for desfuroylceftiofur. Moreover, all tetracyclines, 3 out of 5 sulfonamides and also tylosin, tilmicosin, gentamycin, neomycin, lincomycin and pirlimycin were detected at their respective MRL. The repeatability in scores for the Charm Blue Yellow II is not always as good as the repeatability in colour factors obtained for other similar microbiological tests. Interferences (false positive results) were observed for high somatic cell milk, for milk with a high fat content and for milk with a low or high protein content. These results are consistent with other inhibitory tests evaluated, with the exception of CMT (Copan Milk Test).

M3 - C1: Articles in proceedings

SN - 978-94-6173-318-4

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SP - 563

EP - 568

BT - Residues of veterinary drugs in food

A2 - Schilt, Robert

T2 - EuroResidue VII Conference on Residues of Veterinary Drugs in Food

Y2 - 14 May 2012 through 16 May 2012

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Validation of the microbiological inhibitor test Charm Blue Yellow II for screening for antimicrobials in milk

Uittreksel

Projecten

VALIDATIEANTIBIO: Validatie van screeningstesten voor antibioticaresiduen voor kitproducenten

DETTECH: Ontwikkeling en validatie van nieuwe methoden voor detectie van residuen.

Dit citeren