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A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip GMO, Eppendorf, Germany).
The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. TheGMOconcentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance
criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy
rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and
suitable as a screening tool.
The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. TheGMOconcentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance
criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy
rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and
suitable as a screening tool.
Oorspronkelijke taal | Engels |
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Tijdschrift | European Food Research and Technology |
Aantal pagina’s | 12 |
Publicatiestatus | Gepubliceerd - 2008 |
Vingerafdruk
Bekijk de onderzoeksthema's van 'Validation of the performance of a GMO multiplex screening assay based on microarray detection'. Samen vormen ze een unieke vingerafdruk.Projecten
- 2 Afgerond
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GMODETEC: Ontwikkeling van een algemene strategie voor detectie, identificatie en kwantificatie van genetisch gemodificeerd materiaal in voedingsproducten en veevoeders
Taverniers, I. (Onderzoeker), De Loose, M. (Projectverantwoordelijke) & Ruttink, T. (Voormalig Onderzoeker)
1/01/07 → 31/12/10
Project: Onderzoek
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CO-EXTRA: GG- (genetisch gewijzigde) en niet-GG productieketens: hun co-existentie en traceerbaarheid
Taverniers, I. (Projectbegeleider) & De Loose, M. (Projectverantwoordelijke)
1/01/05 → 31/12/09
Project: Onderzoek