TY - JOUR
T1 - Variation of diazinon and amitraz susceptibility of Hyalomma marginatum (Acari: Ixodidae) in the Rabat-Sale-Kenitra region of Morocco
AU - Elhachimi, Latifa
AU - Van Leeuwen, Thomas
AU - Dermauw, Wannes
AU - Rogiers, Carolien
AU - Valcárcel, Félix
AU - Olmeda, Angeles Sonia
AU - Khatat, Sarah Elhamiani
AU - Daminet, Sylvie
AU - Sahibi, Hamid
AU - Duchateau, Luc
N1 - Copyright © 2021 The Author(s). Published by Elsevier GmbH.. All rights reserved.
PY - 2022/3
Y1 - 2022/3
N2 - In the present study, the acaricide susceptibility status of Hyalomma marginatum in Morocco was investigated in the Rabat-Sale-Kenitra region using the Larval Packet Test. The overall LC50 value for diazinon and amitraz was 115 ppm (95% CI: [104; 125]) and 22 ppm (95% CI: [21; 23]), respectively. The LC50 values varied significantly between the nine sampled locations (P<0.001) ranging from 75 ppm (95% CI: [65; 84]) in Ouelmes to 179 ppm (95% CI: [139; 201]) in Jorf El Melha for diazinon and from 18 ppm (95% CI: [15; 21]) in Skhirat to 28 ppm (95% CI: [24; 31]) in Ouelmes for amitraz. Sequencing of the target-site of diazinon, acetylcholinesterase 1 (AChE1), indicated that previously reported resistance mutations in AChE1 were absent in ticks from Jorf El Melha surviving 500 ppm diazinon. This study is the first report on the H. marginatum susceptibilty status to the most frequently used acaricides in Morocco and indicates that acaricide tick resistance is emerging.
AB - In the present study, the acaricide susceptibility status of Hyalomma marginatum in Morocco was investigated in the Rabat-Sale-Kenitra region using the Larval Packet Test. The overall LC50 value for diazinon and amitraz was 115 ppm (95% CI: [104; 125]) and 22 ppm (95% CI: [21; 23]), respectively. The LC50 values varied significantly between the nine sampled locations (P<0.001) ranging from 75 ppm (95% CI: [65; 84]) in Ouelmes to 179 ppm (95% CI: [139; 201]) in Jorf El Melha for diazinon and from 18 ppm (95% CI: [15; 21]) in Skhirat to 28 ppm (95% CI: [24; 31]) in Ouelmes for amitraz. Sequencing of the target-site of diazinon, acetylcholinesterase 1 (AChE1), indicated that previously reported resistance mutations in AChE1 were absent in ticks from Jorf El Melha surviving 500 ppm diazinon. This study is the first report on the H. marginatum susceptibilty status to the most frequently used acaricides in Morocco and indicates that acaricide tick resistance is emerging.
U2 - 10.1016/j.ttbdis.2021.101883
DO - 10.1016/j.ttbdis.2021.101883
M3 - Article
C2 - 34894524
SN - 1877-959X
VL - 13
SP - 101883
JO - Ticks and tick-borne diseases
JF - Ticks and tick-borne diseases
IS - 2
ER -