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Uittreksel
Red clover (Trifolium pratense L.) is an
important perennial forage crop in Europe. Unfortunately,
seed production of modern cultivars is often
unsatisfactory and breeding progress for higher seed
yield is slow. We evaluated whether marker assisted
parentage analysis could identify progeny plants with
two high seed-yielding parents in a diploid selection
trial. Furthermore, we studied if breeding for high seed
yield could be speeded up by selecting progeny plants
with two high seed-yielding parents, compared to
traditional selection on the mother plant only. In this
study, 111 genotypes with excellent vegetative traits
from a first cycle selection trial were allowed to
pollinate each other in isolation. After seed harvest,
ten high seed-yielding plants were identified and their
seeds were used to grow ten half-sib progeny populations,
with 1 121 plants in total. Two multiplex
primer sets targeting 18 SSR loci were designed and
used to fingerprint parental and progeny plants.
Parentage was reliably determined for 1 083 progeny
plants: 135 plants were the result of a cross between
two high seed-yielding parents. In a 2-year seed yield
trial, the seed yield of these 135 progeny plants was
compared to that of a control group of 216 progeny
plants from the same ten high seed-yielding mother
plants but with no selection on the male contribution,
as is current practice. Compared to the control group,
progeny plants with two high seed-yielding parents
had significantly higher seed yields, 23.0 and 75.9 %
higher in 2012 and 2013 respectively, and a significantly
higher thousand seed weight, 6.6 and 5.7 %
higher in 2012 and 2013 respectively. Seed yield was
strongly correlated with flower abundance (r = 0.643)
and plant volume (r = 0.593 in 2012 and r = 0.509 in
2013) and negatively correlated with susceptibility to
mildew (r = -0.145). We conclude that breeding
programs aiming at increasing seed yield in diploid red
clover cultivars would strongly benefit from the
integration of marker assisted parentage analysis to
identify those progeny plants that are the result of a
cross between two high seed-yielding plants. This goal
cannot be achieved with traditional family selection
where only the seed yield of the mother plant is
known.
Taal | Engels |
---|---|
Tijdschrift | Euphytica |
Volume | 200 |
Exemplaarnummer | 2 |
Pagina's (van-tot) | 305-320 |
Aantal pagina's | 16 |
ISSN | 0014-2336 |
DOI's | |
Status | Gepubliceerd - 2014 |
Trefwoorden
- B390-veredeling
Vingerafdruk Bekijk de onderzoeksthema's van 'Verbetering van zaadopbrengst in rode klaver door merkergeassisteerde vaderschapsanalyse'. Samen vormen ze een unieke vingerafdruk.
Projecten
- 1 Actief