Vergelijken van zaadopbrengst bij mitotische en meiotische rode klaver planten

    Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

    Uittreksel

    New tetraploids can be created by chromosome doubling (mitotic tetraploids), or through unreduced gametes (meiotic tetraploids). In this study, meiotic tetraploids were generated in interploidy crosses, their seed yield was compared against mitotic tetraploids with similar genetic background, and the relation between genetic diversity and seed yield was explored. A total of 15 meiotic tetraploids were obtained in 16 interploidy crosses between diploid Lemmon or Merian genotypes and tetraploid Elanus genotypes. Mitotic tetraploids were created by crossing plants from tetraploidized Lemmon or Merian populations with Elanus genotypes. Tetraploid progeny from cross-pollinations were identified with flow cytometry and SSR marker analysis. The efficiency of interploidy crosses to generate meiotic tetraploids was low (0.5% in diploid and 0.7% tetraploid mother plants) and the degree of self-pollination was high (95.0% in diploid and 94.1% in tetraploid mother plants). Using seeds from the diploid parent was more time and cost efficient because most undesired progeny was identified by flow cytometry, leaving fewer plants to be screened with SSR markers. Meiotic tetraploids yielded significantly more seeds than mitotic tetraploids with similar genetic background (15.9g vs. 10.4 g), but their genetic diversity was not higher (2.6 vs. 2.9 alleles per SSR locus). Seed yield and the average allele number per locus were not correlated among all progeny plants.
    TaalEngels
    TitelBreeding Grasses and Protein Crops in the Era of Genomics
    EditorsGintaras Brazauskas, Grazina Statkeviciute, Kristina Jonaviciene
    UitgeverijSpringer International Publishing Switzerland
    Datum2018
    Pagina's39-45
    ISBN van geprinte versie978-3-319-89577-2
    StatusGepubliceerd - 2018
    EvenementBreeding Grasses and Protein Crops in the Era of Genomics: 32nd Meeting of the Section "Forage Crops and Amenity Grasses" of Eucarpia - Crowne Plaza Hotel, Vilnius, Litouwen
    Duur: 17-sep-201721-sep-2017
    Congresnummer: 32

    Trefwoorden

    • B390-veredeling

    Vingerafdruk

    Bekijk de onderzoeksthema's van 'Vergelijken van zaadopbrengst bij mitotische en meiotische rode klaver planten'. Samen vormen ze een unieke vingerafdruk.

    Dit citeren